Role of the ancillary ligands on the stabilization of the imino-oxo tautomer of 1-methylcytosine in Pt(II) complexes.

The mixed nucleobases complexes cis-[L(2)Pt{1-MeTy(-H)}(1-MeCy,N(3))]NO(3) (L = PPh(3), ; PMePh(2), ), containing the N(3)-deprotonated 1-methylthymine (1-MeTy(-H)) and the neutral 1-methylcytosine (1-MeCy) have been prepared and characterised. The compounds were obtained by reacting the hydroxo complexes cis-[L(2)Pt(mu-OH)](2)(NO(3))(2) with 1-methylthymine (1-MeTy), followed by the addition of 1 equivalent of 1-MeCy. In solution of DMSO, DMF or chlorinated solvents, converts quantitatively into the isomer cis-[L(2)Pt{1-MeTy(-H)}(1-MeCy,N(4))]NO(3) () containing the tautomeric form of the cytosine stabilized through the coordination at the N(4) atom, as shown by single-crystal X-ray analysis. The structural determination of shows the presence in the unit cell of two crystallographic independent complexes having similar conformation, with a different orientation of the two nucleobases (head-head and head-tail) according to the presence of both isomers in solution. Complex , having the less hindered PMePh(2) ligands, in DMSO solution, contains the tautomeric forms of the cytosine in equilibrium and the migration of the metal from the N(3) to N(4) site occurs only to a minor extent.